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Molecular Biotechnology and Gene Therapy

Prof. Dr. Christian BuchholzProf. Dr. Christian Buchholz Source: PEI

Team

Dr. Tobias Abel (Pharmacist)
Dr. Jessica Hartmann (Biochemist)
Dr. Alexander Muik (Biochemist)
Dr. Irene Schneider (Biochemist)
Dr. Qi Zhou (Microbiologist)
Ruben Bender (Pharmacist, PhD student)
Thorsten Friedel (Biochemist, PhD student)
Julia Hanauer (Biochemist, PhD student)
Dina Kleinlützum (Veterinarian, doctoral student)
Anke Muth (Biochemist, PhD Student)
Anett Pfeiffer (Biologist, PhD student)
Johanna Reul (Biochemist, PhD student)
Ruth Freiling (Bachelor Scientist)
Patricia Hartmann (Bachelor Scientist)
Gundula Braun (Technician)
Julia Brynza (Technician)
Manuela Schütze (Technician)

Research Summary

Surface engineering of viral gene transfer vectors

AAV precision gene vectors: The receptor binding target structures (DARPins) are shown in red, yellow, or green. These structures guide the gene vectors to the therapy relevant cells.AAV precision gene vectors: The receptor binding target structures (DARPins) are shown in red, yellow, or green. These structures guide the gene vectors to the therapy relevant cells. Source: PEI

Viruses have evolved as optimally adapted particles to enter cells and transfer their genetic material. We are engineering the surface of enveloped virus particles to improve their applicability in modern molecular medicine. In particular we are following the hypothesis that viral gene transfer vectors and oncolytic viruses engineered to use a cell surface receptor of choice instead of their natural receptor will improve in vivo gene transfer and will pave the road to novel therapeutic options. As delivery and display vehicle we are using lentiviruses (LVs), adenovirus-associated virus (AAV), measles virus (MV), and vesicular stomatitis virus (VSV) each offering distinct beneficial properties.
The projects include basic work on the technology as well as applications of the generated vector systems for gene function studies as well as for therapeutic options focussing on the treatment of malignant tumours and neurodegenerative diseases.

Summary of ongoing research projects

  1. Improving the technology of receptor targeting
  2. Tumour and tumor stem cell targeting
  3. Targeted gene transfer to subtypes of lymphocytes
  4. Gene transfer to the central nervous system

10 Most Important Publications (2010-2015)

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Additional Information

Further Information

Precise gene transfer into therapy relevant cells after vector injection into blood

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